Dr. Tan Joo Shun
School of Industrial Technology
Email : jooshun@usm.my
Phone No. : +6(04) 653 (Ext) 6376
Objectives
- To investigate the role of polymer that impregnated into resin on the interaction mechanism of polymer and two different protein species, bacteriocin and lipase by measuring partition coefficient.
- To investigate the extraction behaviors of two different protein species, bacteriocin and lipase, to focus on impregnation in terms of stability and phase system via protein-impregnated resin interactions in TAPPIR system.
- To show the effect of extraction process parameters of TAPPIR system on two different proteins, bacteriocin and lipase, using response surface methodology and artificial neural network for enhancement of the performance of TAPPIR system in combination with flotation system.
Impact To Goverment / Industry / Academic / Society
Tunable Aqueous Polymer-Phase Impregnated Resins Technology
Impregnation of dyed PEG into XAD4 resins
1) One phase of polymer
2) Polymer is being adsorbed into the Amberlite XAD4
3) The Amberlite XAD4 is fully impregnated with PEG
Recovery of bovine serum albumin (BSA) using AIRS
Polymer phase impregnation and extraction behavior of protein
AQUEOUS TWO PHASE SYSTEM
In ATPS, purification fold of 3.4 and recovery yield of 93% were obtained for lipase protein.
TAPPIR
In TAPPIR, purification fold of 7 .6 and recovery yield of 89% were obtained for lipase protein.